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Bud blight is an emerging viral problem in tomato. Bud blight
affected plants reacted positively to polyclonal antiserum of
Groundnut Bud necrosis Virus (N gene). TN-Co, UP-Ka, MH-Pu and
MH-Ra isolates could infect 13 out of 27 plant species tested
belonging to Solanaceae, Leguminoase and Malvaceae. The
nucleocapsid protein (N) and movement protein (NSm) genes were
cloned and sequenced using GBNV specific primers. N gene of the
tomato Tospovirus isolates shared 97-100% amino acid sequence
identity with GBNV (type isolate). Further the N gene among GBNV
isolates originating from tomato and other hosts and different
locations was highly conserved (96-99%), suggesting their common
origin. Similarly the NSm genes of tomato Tospovirus isolates from
different locations shared 98% amino acid sequence identity with
GBNV (type isolate) and were highly conserved (96-98%). N gene from
GBNV TN-Co isolate was used as transgene to confer resistance in
tomato. Nine transgenic lines Co3S1, Co3S2, Co3S12, PRS1, PRS3,
PRA1, PRA2, PRA4 and PRA5 were Southern positive. None of the T0
lines showed symptoms upon mechanical inoculation with TN-Co
isolate
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